The lawsuit claims the actions by the men contributed to Spierer's "disappearance, injury and death" and seeks unspecified compensation.
No arrests have been made in Spierer's disappearance and there's been no sign of her despite numerous searches around Bloomington and parts of the surrounding wooded countryside that's dotted with old limestone quarries and lakes.
Attorney Jason Barclay said Robert and Charlene Spierer reluctantly filed the lawsuit on May 31 in Monroe County court because they faced a legal deadline with the two-year mark since their daughter's disappearance, The Herald-Times and The Indianapolis Star reported.
"Any parent in search of information about a missing child would use every resource available to them," Barclay said. "Therefore, we intend to use the rights afforded by the civil justice system to obtain answers to questions that have gone unanswered for too long. We fully expect that those with relevant information will cooperate with this process."
Messages for the three students' attorneys weren't immediately returned Thursday.
The Spierers have maintained that several of the people who were with her that night haven't fully cooperated with investigators. Bloomington police officials won't comment on the search other than to say the department has received more than 3,000 tips.
The Spierers' lawsuit was transferred last week to federal court at the request of lawyers for the three men, in part because they all now live outside Indiana.
The lawsuit alleges that at various times that night the men provided her with alcohol at apartments and a downtown bar and then let her walk alone to her apartment building several blocks away about 4:30 a.m.